KMID : 0352720210450040490
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Journal of Ginseng Research 2021 Volume.45 No. 4 p.490 ~ p.497
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Ginsenoside Rk1 suppresses platelet mediated thrombus formation by downregulation of granule release and ¥áIIb¥â3 activation
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Shin Jung-Hae
Kwon Hyuk-Woo Irfan Muhammad Rhee Man-Hee Lee Dong-Ha
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Abstract
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Background and objective: Synthetic ginsenoside compounds G-Rp (1,3, and 4) and natural ginsenosides in Panax ginseng 20(S)-Rg3, Rg6, F4 and Ro have inhibitory actions on human platelets. However, the inhibitory mechanism of ginsenoside Rk1 (G-Rk1) is still unclear thus, we initiated investigation of the anti-platelet mechanism by G-Rk1 from Panax ginseng.
Methodology: Our study focused to investigate the action of G-Rk1 on agonist-stimulated human platelet aggregation, inhibition of platelet signaling molecules such as fibrinogen binding with integrin ¥áIIb¥â3 using flow cytometry, intracellular calcium mobilization, fibronectin adhesion, dense granule secretion, and thromboxane B2 secretion. Thrombin-induced clot retraction was also observed in human platelets.
Key Results: Collagen, thrombin, and U46619-stimulated human platelet aggregation were dose-dependently inhibited by G-Rk1, while it demonstrated a more effective suppression on collagen-stimulated platelet aggregation using human platelets. Moreover, G-Rk1 suppressed collagen-induced elevation of Ca2+ release from endoplasmic reticulum, granule release, and ¥áIIb¥â3 activity without any cytotoxicity.
Conclusions and implications: These results indicate that G-Rk1 possess strong anti-platelet effect, proposing a new drug candidate for treatment and prevention of platelet-mediated thrombosis in cardiovascular disease.
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KEYWORD
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Anti-platelet, Ginsenoside-Rk1, Integrin ¥áIIb¥â3, Thrombosis, Clot retraction
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